Title : Mammalian Crumbs3 is a small transmembrane
protein linked to
protein associated with
Lin-7 (
Pals1 )
Abstract :
- Drosophila Crumbs is a transmembrane protein that plays an important role in epithelial cell polarity and photoreceptor development
- Overexpression of Crumbs in Drosophila epithelia expands the apical surface and leads to disruption of cell polarity
- Drosophila Crumbs also interacts with two other polarity genes, Stardust and Discs Lost
- Recent work has identified a human orthologue of Drosophila Crumbs, known as CRB1 , that is mutated in the eye disorders, retinitis pigmentosa and Leber congenital amaurosis
- Our work has demonstrated that human CRB1 can form a complex with mammalian orthologues of Stardust and Discs Lost, known as protein associated with Lin-7 ( Pals1 ) and Pals1 associated tight junction ( PATJ ), respectively
- In the current report we have cloned a full length cDNA for a human paralogue of CRB1 called Crumbs3 ( CRB3 )
- In contrast to Drosophila Crumbs and CRB1 , CRB3 has a very short extracellular domain but like these proteins it has a conserved intracellular domain that allows it to complex with Pals1 and PATJ
- Mouse and human CRB3 have identical intracellular domains but divergent extracellular domains except for a conserved N-glycosylation site
- CRB3 is localized to the apical surface and tight junctions but the conserved N linked glycosylation site does not appear to be necessary for CRB3 apical targeting
- CRB3 is a specialized isoform of the Crumbs protein family that is expressed in epithelia and can tie the apical membrane to the tight junction
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. linked, , protein, Mammalian Crumbs3, -
- 8. N-glycosylation, , -, -, site
- 9. glycosylation, , -, -, site
Output(Part-Of) (sent_index,
protein,
site):
- 7. CRB3, domain
- 8. CRB3, domains
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):