PMID: 12545205

 

    Legend: Gene, Sites

Title : Carbohydrate moieties of N-cadherin from human melanoma cell lines

Abstract :
  1. Expression of N-cadherin an adhesion molecule of the cadherin family, in tumor cells is associated with their increased invasive potential
  2. Many studies suggested the role of N-linked oligosaccharides as important factors that contribute to metastasis by influencing tumor cell invasion and adhesion
  3. N-cadherin is a heavily glycosylated protein
  4. We have analysed the carbohydrate profile of this protein synthesized in human melanoma cell lines: WM35 from the primary tumor site and WM239, WM9, and A375 from different metastatic sites
  5. N-cadherin was immunoprecipitated with anti-human N-cadherin polyclonal antibodies
  6. Characterisation of its carbohydrate moieties was carried out by SDS /PAGE electrophoresis and blotting, followed by immunochemical identification of the N-cadherin polypeptides and analysis of their glycans using highly specific digoxigenin or biotin labelled lectins
  7. The positive reaction of N-cadherin from the WM35 cell line with Galanthus nivalis agglutinin ( GNA ), Datura stramonium agglutinin ( DSA ) and Sambucus nigra agglutinin ( SNA ) indicated the presence of high-mannose type glycans and biantennary complex type oligosaccharides with alpha2-6 linked sialic acid
  8. N-cadherin from WM239, WM9, and A375 cell lines gave a positive reaction with Phaseolus vulgaris leukoagglutinin ( L-PHA ) and lotus Tetragonolobus purpureas agglutinin ( LTA )
  9. This indicated the presence of tri- or tetra-antennary complex type glycans with alpha-fucose
  10. In addition, N-cadherin from WM9 (lymphomodus metastatic site ) and A375 (solid tumor metastatic site ) contained complex type chains with alpha2-3 sialic acid (positive reaction with Maackia amurensis agglutinin--MAA)
  11. The results demonstrated that N-glycans of N-cadherin are altered in metastatic melanomas in a way characteristic for invasive tumor cells
Output (sent_index, trigger, protein, sugar, site):
  • 0. N-cadherin, , N-cadherin, Carbohydrate moieties, -
  • 10. contained, , N-cadherin, complex type chains, -
  • 11. N-cadherin, , N-cadherin, N-glycans, -
  • 3. glycosylated, , N-cadherin, -, -
  • 3. glycosylated, , protein, -, -
  • 4. carbohydrate, , protein, -, -
  • 4. protein, , protein, the carbohydrate profile, -
Output(Part-Of) (sent_index, protein, site):
  • 6. N-cadherin, polypeptides
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
cadherin 1000 1
N-cadherin 1000 0,1,3,5,6,7,8,10,11