PMID: 12654314

 

    Legend: Gene, Sites

Title : Site-specific characterization of the N-linked oligosaccharides of a murine immunoglobulin M by high-performance liquid chromatography/electrospray mass spectrometry

Abstract :

1. Immunoglobulin M is an especially important product of the immune system because it plays a critical role in early protection against infections
2. In this report, the glycosylation pattern of the protective murine monoclonal IgM 12A1 to Cryptococcus neoformans polysaccharide was analyzed by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry
3. Peptide mapping studies covering 88% of the deduced amino acid sequence indicated that of the six potential N-glycosylation sites in this antibody only five were utilized, as the tryptic peptide derived from monoclonal IgM 12A1 containing Asn-260 was recovered without carbohydrates
4. The oligosaccharide side chains of monoclonal IgM 12A1 were characterized at each of the N-glycosylation sites
5. Asn-166 possessed 20 monosialylated and nonsialylated, and fucosylated and nonfucosylated complex- and hybrid-type oligosaccharides and one high-mannose-type oligosaccharide
6. Thirteen oligosaccharides were attached to the site at Asn-401 , including six complex-type, four hybrid-type, and three high-mannose-type oligosaccharides
7. Twelve hybrid-type oligosaccharides were attached to Asn-378 , three of which had terminal sialic acids
8. Eleven hybrid-type oligosaccharides were attached to Asn-331 , seven of which had terminal sialic acids
9. Only two high-mannose type oligosaccharides were attached to Asn-363
10. These results indicated great complexity in the structure and com position of oligosaccharides attached to individual IgM glycosylation sites

Output (sent_index, trigger, protein, sugar, site):

• 0. M, , immunoglobulin M, the N-linked oligosaccharides, -
• 10. attached, , -, oligosaccharides, sites
• 10. glycosylation, , -, -, sites
• 3. N-glycosylation, , -, -, sites
• 4. N-glycosylation, , -, -, sites
• 5. possessed, , -, hybrid-type oligosaccharides, Asn-166
• 5. possessed, , -, one high-mannose-type oligosaccharide, Asn-166
• 6. attached, , -, Thirteen oligosaccharides, Asn-401
• 6. attached, , -, Thirteen oligosaccharides, site
• 7. attached, , -, Twelve hybrid-type oligosaccharides, Asn-378
• 7. had, , -, terminal sialic acids, Asn-378
• 8. attached, , -, Eleven hybrid-type oligosaccharides, Asn-331
• 8. had, , -, terminal sialic acids, Asn-331
• 9. attached, , -, Only two high-mannose type oligosaccharides, Asn-363

Output(Part-Of) (sent_index, protein, site):

• 10. IgM, sites

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 5. immunoglobulin M, hybrid-type oligosaccharides, Asn-166
• 5. immunoglobulin M, one high-mannose-type oligosaccharide, Asn-166
• 6. immunoglobulin M, Thirteen oligosaccharides, Asn-401
• 7. immunoglobulin M, Twelve hybrid-type oligosaccharides, Asn-378
• 7. immunoglobulin M, terminal sialic acids, Asn-378
• 8. immunoglobulin M, Eleven hybrid-type oligosaccharides, Asn-331
• 8. immunoglobulin M, terminal sialic acids, Asn-331
• 9. immunoglobulin M, Only two high-mannose type oligosaccharides, Asn-363

 

Protein	NCBI ID	SENTENCE INDEX