PMID: 12775711

 

    Legend: Gene, Sites

Title : Structure and ligand-induced conformational change of the 39-kDa glycoprotein from human articular chondrocytes

Abstract :

1. The 39-kDa human cartilage glycoprotein ( HCGP39 ), a member of a novel family of chitinase-like lectins (Chilectins), is overexpressed in articular chondrocytes and certain cancers
2. Proposed functions of this protein include a role in connective tissue remodeling and defense against pathogens
3. Similar to other Chi-lectins, HCGP39 promotes the growth of connective tissue cells
4. The ability of HCGP39 to activate cytoplasmic signaling pathways suggests the presence of a ligand for this protein at the cell surface
5. There is currently no information regarding the identity of any physiological or pathological ligands of the Chi-lectins or the nature of the protein-ligand interaction
6. Here, we show that HCGP39 is able to bind chitooligosaccharides with micromolar affinity
7. Crystal structures of the native protein and a complex with GlcNAc8 show that the ligand is bound in identical fashion to family 18 chitinases
8. However, unlike the chitinases, binding of the oligosaccharide ligand to HCGP39 induces a large conformational change
9. Thus, HCGP39 could be a lectin that binds chitin-like oligosaccharide ligands and possibly plays a role in innate responses to chitinous pathogens, such as fungi and nematodes

Output (sent_index, trigger, protein, sugar, site):

• 0. glycoprotein, , glycoprotein, -, -
• 1. glycoprotein, , HCGP39, -, -
• 1. glycoprotein, , glycoprotein, -, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
Chi-lectins, HCGP39	1116	3
HCGP39	1116	1,4,6,8
Thus, HCGP39	1116	9