PMID: 12839991

 

    Legend: Gene, Sites

Title : Structure and axon outgrowth inhibitor binding of the Nogo-66 receptor Nogo-66 receptor and related proteins

Abstract :

1. The myelin-derived proteins Nogo , MAG and OMgp limit axonal regeneration after injury of the spinal cord and brain
2. These cell-surface proteins signal through multi- subunit neuronal receptors that contain a common ligand-binding glycosylphosphatidylinositol-anchored subunit termed the Nogo-66 receptor Nogo-66 receptor ( NgR )
3. By deletion analysis, we show that the binding of soluble fragments of Nogo , MAG and NgR to cell-surface NgR requires the entire leucine-rich repeat (LRR) region of NgR , but not other portions of the protein
4. Despite sharing extensive sequence similarity with NgR , two related proteins , NgR2 and NgR3 , which we have identified, do not bind Nogo , MAG , OMgp or NgR
5. To investigate NgR specificity and multi-ligand binding, we determined the crystal structure of the biologically active ligand-binding soluble ectodomain of NgR
6. The molecule is banana shaped with elongation and curvature arising from eight LRRs flanked by an N-terminal cap and a small C-terminal subdomain
7. The NgR structure analysis, as well as a comparison of NgR surface residues not conserved in NgR2 and NgR3 , identifies potential protein interaction sites important in the assembly of a functional signaling complex

Output (sent_index, trigger, protein, sugar, site):

• 3. NgR, , NgR, the entire leucine-rich repeat (LRR) region, -
• 3. leucine-rich, , -, LRR, leucine
• 3. leucine-rich, , -, leucine-rich repeat, leucine

Output(Part-Of) (sent_index, protein, site):

• 3. MAG, fragments
• 3. NgR, fragments
• 3. Nogo, fragments
• 5. NgR, ectodomain
• 7. NgR, residues

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX