PMID: 1323463

 

    Legend: Gene, Sites

Title : Characterisation by mass spectrometry and 1H-NMR of novel hexasaccharides among the acidic O-linked carbohydrate chains of bovine submaxillary mucin

Abstract :

1. The acidic oligosaccharide alditols released from bovine submaxillary-gland mucin by Carlson degradation were investigated by a combination of liquid secondary-ion mass spectrometry, methylation analysis and 1H-NMR
2. Among the largest structures identified were four branched hexasaccharides, three of them novel, comprising two separate pairs of structures
3. One pair contained the sequence Fuc(alpha 1-2)Gal(beta 1-4)[Fuc(alpha 1-3)]GlcNAc(beta 1-) (Fuc, L-fucose), at C3 of N-acetylgalactosaminitol and differed only by substitution at C6 by N-acetylneuraminic or N-glycolylneuraminic acid
4. The other pair also differed in substitution of the sialic acid linked at C6 and contained the GalNAc-(alpha 1-3)[Fuc(alpha 1-2)]Gal(beta 1-4)GlcNAc(beta 1-), sequence at C3 of N-acetylgalactosaminitol
5. The Lewis(y) and blood-group-A determinants of these sequences have not been found previously in the acidic oligosaccharides of bovine submaxillary-gland mucin , although they have recently been characterised in the neutral chains of bovine submaxillary-gland mucin

Output (sent_index, trigger, protein, sugar, site):

• 0. mucin, , mucin, the acidic O-linked carbohydrate chains, -
• 1. released, , mucin, The acidic oligosaccharide alditols, -
• 5. mucin, , mucin, the acidic oligosaccharides, -
• 5. sequences, , -, The Lewis(y) and blood-group-A determinants, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
mucin	281333	0,1,5