PMID: 1421756

 

    Legend: Gene, Sites

Title : Presence of O-linked oligosaccharide on a threonine residue in the human transferrin receptor

Abstract :
  1. We have previously demonstrated that the human transferrin receptor ( TfR ) of approximately 90 kDa contains Ser/Thr-linked (O-linked) oligosaccharides
  2. In the present study, we report our identification of the site of attachment of the O-linked oligosaccharides in the receptor
  3. A 70 kDa fragment from the external domain of the TfR was generated by trypsin treatment of the [3H]glucosamine-labelled receptor purified from human K562 cells
  4. The beta-elimination of the intact TfR , but not the 70 kDa fragment , released Gal-[3H]Gal-NAcitol, indicating that the 70 kDa fragment lacks O-linked oligosaccharides
  5. In the remaining 20 kDa fragment there are three potential sites ( Thr96, Thr104 and Ser106 ) for O-glycosylation in the extracellular domain
  6. To identify which of these residues are O-glycosylated, both the [3H] Thr- and [3H] Ser-labelled TfR were directly treated with mild base to effect beta-elimination, and the radiolabelled amino acids and their derivatives were analysed
  7. Approximately 2% of the total radiolabelled Thr , but no radiolabelled Ser , was converted to expected beta-elimination products by this treatment
  8. These and other results demonstrate that only one O-linked oligosaccharide is present in the TfR and that it occurs on either Thr96 or Thr104
  9. From human serum we purified the cleaved, soluble form of the TfR (s- TfR ), which contains Thr104 , but lacks Thr96
  10. The s- TfR was sensitive to O-glycanase and bound to Jacalin lectin , indicating that the s- TfR contains an O-linked oligosaccharide
  11. (ABSTRACT TRUNCATED AT 250 WORDS
Output (sent_index, trigger, protein, sugar, site):
  • 1. contains, , TfR, Ser/Thr-linked (O-linked) oligosaccharides, -
  • 1. contains, , transferrin receptor, Ser/Thr-linked (O-linked) oligosaccharides, -
  • 10. contains, , TfR, an O-linked oligosaccharide, -
  • 2. oligosaccharides, , receptor, oligosaccharides, -
  • 8. present, , TfR, only one O-linked oligosaccharide, -
Output(Part-Of) (sent_index, protein, site):
  • 0. transferrin receptor, threonine residue
  • 3. -, fragment
  • 3. TfR, domain
  • 9. TfR, Thr104
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
transferrin receptor 7037 0,1