Title :
Hepatocyte growth factor is linked by O-glycosylated oligosaccharide on the alpha chain
Abstract :
- The glycosylation site and the structure of O-glycosylated oligosaccharide of recombinant human HGF were investigated
- N-acetylgalactosamine (GalNAc) in the alpha chain suggested the presence of O-glycosylated oligosaccharide
- Sugar analysis and amino acid sequence analysis of peptide fragments produced by limited degradation revealed that O-glycosylated oligosaccharide linked to Thr445 of the alpha chain
- The molecular weight of the oligosaccharide was determined with ion spray mass spectrometry
- From these studies, the structure of the O-glycosylated oligosaccharide on the alpha chain of HGF was concluded as [formula: see text]
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. O-glycosylated, , -, O-glycosylated oligosaccharide, -
- 1. HGF, , HGF, O-glycosylated oligosaccharide, -
- 1. O-glycosylated, , -, O-glycosylated oligosaccharide, -
- 1. glycosylation, , -, O-glycosylated oligosaccharide, site
- 2. O-glycosylated, , -, O-glycosylated oligosaccharide, -
- 3. O-glycosylated, , -, O-glycosylated oligosaccharide, -
- 3. linked, , -, O-glycosylated oligosaccharide, Thr445
- 5. O-glycosylated, , -, the O-glycosylated oligosaccharide, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 3. Hepatocyte growth factor, O-glycosylated oligosaccharide, Thr445