PMID: 1517205

 

    Legend: Gene, Sites

Title : Human factor IX has a tetrasaccharide O-glycosidically linked to serine 61 through the fucose residue

Abstract :

1. We have recently discovered unusual sugar chains (xylose (Xyl)-glucose (Glc) and (Xyl)2-Glc) linked to a serine residue in the epidermal growth factor ( EGF )-like domains of human and bovine clotting factors VII ( Ser-52 ), IX ( Ser-53 ), and protein Z ( Ser-53 ), in addition to bovine platelet glycoprotein thrombospondin
2. We now have evidence of another modification in the first EGF-like domain of human factor IX , which proved to be a tetrasaccharide O-fucosidically linked to Ser-61
3. Two large peptides containing Ser-61 ( positions 44-63), named hIX-GP1 and hIX-GP2, were first isolated from the lysyl endopeptidase-digest of human factor IX , by reversed-phase high performance liquid chromatography (HPLC)
4. Data on the component sugar analysis after pyridylamination (PA) and sialic acid analysis of the isolated peptides indicated that they contained 1 mol each of galactose ( Gal ), fucose (Fuc), N-acetylglucosamine (GlcNAc), and N-acetylneuraminic acid (NeuAc), in addition to Glc and Xyl
5. hIX-GP1 was further digested with asparaginyl endopeptidase , and two glycopeptides containing Ser-61 , named N-3 ( positions 59-63) and N-9 ( positions 55-63), were isolated, respectively
6. These glycopeptides were both composed of 1 mol each of Gal, Fuc, GlcNAc, and NeuAc but did not contain Xyl and Glc
7. Moreover, the data on beta-elimination for N-9 and of the fast atom bombardment mass spectrometric analysis on peptide N-3 suggested the presence of a tetrasaccharide linked to Ser-61
8. An analysis of the PA-oligosaccharide released from hIX-GP1 by hydrazinolysis followed by pyridylamination revealed that the reducing end was PA-Fuc
9. All the results support the proposal that human factor IX has a novel tetrasaccharide consisting of 1 mol each of Gal , Fuc, GlcNAc, and NeuAc, which is O-glycosidically linked to Ser-61 through the Fuc residue

Output (sent_index, trigger, protein, sugar, site):

• 0. has, , factor IX, a tetrasaccharide, -
• 0. linked, , -, a tetrasaccharide, serine 61
• 1. glycoprotein, , glycoprotein, -, -
• 1. linked, , -, (Xyl)2-Glc, serine residue
• 2. factor, , factor IX, a tetrasaccharide O-fucosidically, -
• 2. linked, , -, a tetrasaccharide O-fucosidically, Ser-61
• 4. peptides, , -, sialic acid analysis, peptides
• 5. glycopeptides, , -, -, glycopeptides
• 5. positions, , -, -, positions 55
• 5. positions, , -, -, positions 59
• 6. contain, , -, Glc, glycopeptides
• 6. contain, , -, Xyl, glycopeptides
• 6. glycopeptides, , -, -, glycopeptides
• 7. linked, , -, a tetrasaccharide, Ser-61

Output(Part-Of) (sent_index, protein, site):

• 1. factor, domains
• 1. factors, domains
• 1. factors, serine residue
• 1. protein Z, Ser-53
• 2. EGF, domain
• 2. factor IX, domain
• 3. -, Ser-61
• 3. -, positions 44

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 0. factor IX, a tetrasaccharide, serine 61
• 2. factor IX, a tetrasaccharide O-fucosidically, Ser-61
• 7. factor IX, a tetrasaccharide, Ser-61

 

Protein	NCBI ID	SENTENCE INDEX