Title : Glycosylation
site for chondroitin sulfate on the neural part-time proteoglycan, neuroglycan C. Neuroglycan C (
NGC ) is a membrane-spanning chondroitin sulfate (CS) proteoglycan that is expressed predominantly in the central nervous system (CNS)
Abstract :
- NGC dramatically changed its structure from a proteoglycan to a nonproteoglycan form with cerebellar development, whereas a small portion of NGC molecules existed in a nonproteoglycan form in the other areas of the mature CNS, suggesting that the CS glycosylation of NGC is developmentally regulated in the whole CNS
- As primary cultured neurons and astrocytes from cerebral cortices expressed NGC in a proteoglycan form and in a nonproteoglycan form, respectively, CS glycosylation seems to be regulated differently depending on cell type
- To investigate the glycosylation process, cell lines expressing a proteoglycan form of NGC would be favorable experimental models
- When a mouse NGC cDNA was transfected into COS 1, PC12D, and Neuro 2a cells, only Neuro 2a cells, a mouse neuroblastoma cell line, expressed NGC bearing CS chains
- In PC12D cells, although three intrinsic CS proteoglycans were detected, exogenously expressed NGC did not bear any short CS chains just like NGC in the mature cerebellum
- This suggests that the addition of CS chains to the NGC core protein is regulated in a manner different from that of other CS proteoglycans
- As the first step in investigating the CS glycosylation mechanism using Neuro 2a cells, we determined the CS attachment site as Ser-123 on the NGC core protein by site-directed mutagenesis
- The CS glycosylation was not necessary for intracellular trafficking of NGC to the cell surface at least in Neuro 2a cells
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. site, , -, neuroglycan C. Neuroglycan C, site
- 0. site, , -, the neural part-time proteoglycan, site
- 1. glycosylation, , NGC, -, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):