PMID: 15454184

 

    Legend: Gene, Sites

Title : Analysis of site-specific glycosylation in recombinant human follistatin expressed in Chinese hamster ovary cells

Abstract :
  1. Follistatin (FS) FS ), a glycoprotein , plays an important role in cell growth and differentiation through the neutralization of the biological activities of activins
  2. In this study, we analyzed the glycosylation of recombinant human FS ( rhFS ) produced in Chinese hamster ovary cells
  3. The results of SDS-PAGE and MALDI-TOF MS revealed the presence of both non-glycosylated and glycosylated forms
  4. FS contains two potential N-glycosylation sites, Asn95 and Asn259
  5. Using mass spectrometric peptide/glycopeptide mapping and precursor-ion scanning, we found that both N-glycosylation sites were partially glycosylated
  6. Monosaccharide com position analyses suggested the linkages of fucosylated bi- and triantennary complex-type oligosaccharides on rhFS
  7. This finding was supported by mass spectrometric oligosaccharide profiling, in which the m/z values and elution times of some of the oligosaccharides from rhFS were in good agreement with those of standard oligosaccharides
  8. Site-specific glycosylation was deduced on the basis of the mass spectra of the glycopeptides
  9. It was suggested that biantennary oligosaccharides are major oligosaccharides located at both Asn95 and Asn259 , whereas the triantennary structures are present mainly at Asn95
Output (sent_index, trigger, protein, sugar, site):
  • 0. glycosylation, , follistatin, -, -
  • 1. glycoprotein, , Follistatin (FS), -, -
  • 1. glycoprotein, , glycoprotein, -, -
  • 2. glycosylation, , rhFS, -, -
  • 4. N-glycosylation, , -, -, sites, Asn95 and Asn259
  • 5. N-glycosylation, , -, -, sites
  • 5. glycosylated, , -, -, sites
  • 5. peptide/glycopeptide, , -, -, peptide/glycopeptide
  • 6. fucosylated, , -, fucosylated bi- and triantennary complex-type oligosaccharides, -
  • 7. rhFS, , rhFS, the oligosaccharides, -
  • 8. glycopeptides, , -, -, glycopeptides
  • 9. present, , -, the triantennary structures, Asn95
Output(Part-Of) (sent_index, protein, site):
  • 4. FS, sites, Asn95 and Asn259
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 4. FS, -, sites, Asn95 and Asn259
  • 9. follistatin, the triantennary structures, Asn95

 

 

Protein NCBI ID SENTENCE INDEX
Follistatin (FS) 10468 1
follistatin 10468 0