PMID: 15606899

 

    Legend: Gene, Sites

Title : Beta-amyloid protein converting enzyme 1 1 and brain-specific type II membrane protein BRI3 : binding partners processed by furin

Abstract :

1. Using a yeast two-hybrid system, we screened a human brain cDNA library for possible interacting proteins with the C-terminal cytosolic tail of the beta-secretase beta-amyloid protein converting enzyme ( BACE)1
2. This identified seven potential candidates, including the brain-specific type II membrane protein BRI3
3. Co-localization and co-immunoprecipitation experiments confirmed that BACE1 and BRI3 co-localize and interact with each other via the cytosolic tail of BACE1
4. Furthermore, pulse and pulse-chase analyses revealed that the pro- protein convertases furin , and to a lesser extent PC7 and PC5A, process BRI3 into a C-terminal secreted approximately 4-kDa product
5. Thus, furin efficiently processes both pro- BACE1 and its novel interacting protein pro- BRI3

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

• 1. enzyme, tail
• 1. proteins, tail
• 3. BACE1, tail

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
BACE1	23621	3,5
BACE)1	23621	1
BRI3	25798	0,2,3,4,5
Thus, furin	5045	5
PC7	9159	4
furin	5045	0,4