PMID: 15750791

 

    Legend: Gene, Sites

Title : Roles for N-glycosylation in the dynamics of Edg-1 / S1P1 in sphingosine 1-phosphate-stimulated cells

Abstract :

1. Sphingosine 1-phosphate ( Sph-1-P) is a bioactive lipid mediator released from activated platelets
2. To date, 5 seven-transmembrane-spanning receptors , Edg-1 / S1P1 , Edg-3 / S1P3 , Edg-5 / S1P2 , Edg-6 / S1P4 and Edg-8 / S1P5 , have been identified as specific Sph-1-P receptors
3. Our recent novel studies established that Edg-1 / S1P1 is glycosylated in its N-terminal extracellular portion and further identified the specific glycosylation site as asparagine 30
4. We also demonstrated that the structure of the N-terminal ectodomain of Edg-1 / S1P1 affects both its transport to the cell surface and the N-glycosylation process
5. These studies revealed a possible regulatory role for the N-glycan on Edg-1 / S1P1 in the dynamics of the receptor, such as its lateral and internal movements within the membrane, in ligand-stimulated mammalian cells

Output (sent_index, trigger, protein, sugar, site):

• 3. glycosylated, , S1P1, -, -
• 3. glycosylation, , -, -, site
• 5. N-glycan, , Edg-1, N-glycan, -

Output(Part-Of) (sent_index, protein, site):

• 4. Edg-1, ectodomain

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
Edg-8	53637	2
Edg-5	9294	2
S1P4	8698	2
S1P5	53637	2
Edg-3	1903	2
S1P1	1901	0,2,3,4,5
Edg-6	8698	2
S1P3	1903	2
S1P2	9294	2