PMID: 16732286

 

    Legend: Gene, Sites

Title : Crystal structures of the Tie2 receptor ectodomain and the angiopoietin-2- Tie2 complex

Abstract :
  1. The Tie receptor tyrosine kinases and their angiopoietin ( Ang ) ligands play central roles in developmental and tumor-induced angiogenesis
  2. Here we present the crystal structures of the Tie2 ligand-binding region alone and in complex with Ang2
  3. In contrast to prediction, Tie2 contains not two but three immunoglobulin (Ig) domains , which fold together with the three epidermal growth factor domains into a compact, arrowhead-shaped structure
  4. Ang2 binds at the tip of the arrowhead utilizing a lock-and-key mode of ligand recognition-unique for a receptor kinase-where two complementary surfaces interact with each other with no domain rearrangements and little conformational change in either molecule
  5. Ang2- Tie2 recognition is similar to antibody-protein antigen recognition, including the location of the ligand-binding site within the Ig fold
  6. Analysis of the structures and structure-based mutagenesis provide insight into the mechanism of receptor activation and support the hypothesis that all angiopoietins interact with Tie2 in a structurally similar manner
Output (sent_index, trigger, protein, sugar, site):
Output(Part-Of) (sent_index, protein, site):
  • 0. Tie2 receptor, ectodomain
  • 2. Tie2, region
  • 3. Tie2, domains
  • 3. factor, domains
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
angiopoietin-2 285 0
Ang2 285 2,4,5