Title : Identification of
N-linked glycoproteins in human saliva by
glycoprotein capture and mass spectrometry
Abstract :
- Glyco proteins make up a major and important part of the salivary proteome and play a vital role in maintaining the health of the oral cavity
- Because changes in the physiological state of a person are reflected as changes in the glycoproteome com position, mapping the salivary glycoproteome will provide insights into various processes in the body
- Salivary glycoproteins were identified by the hydrazide coupling and release method
- In this approach, glycoproteins were coupled onto a hydrazide resin, the proteins were then digested and formerly N-glycosylated peptides were selectively released with the enzyme PNGase F and analyzed by LC-MS/MS
- Employing this method, coupled with in-solution isoelectric focusing separation as an additional means for pre-fractionation, we identified 84 formerly N-glycosylated peptides from 45 unique N-glycoproteins
- Of these, 16 glycoproteins have not been reported previously in saliva
- In addition, we identified 44 new sites of N-linked glycosylation on the proteins
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycoprotein, , glycoprotein, -, -
- 0. glycoproteins, , glycoproteins, -, -
- 3. glycoproteins, , glycoproteins, -, -
- 4. N-glycosylated, , -, -, peptides
- 4. glycoproteins, , glycoproteins, -, -
- 5. N-glycoproteins, , N-glycoproteins, -, -
- 5. N-glycosylated, , -, -, peptides
- 6. glycoproteins, , glycoproteins, -, -
- 7. glycosylation, , -, -, sites
Output(Part-Of) (sent_index,
protein,
site):
- 5. N-glycoproteins, peptides
- 7. proteins, sites
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):