Title : Modification of
p53 with O-linked N-acetylglucosamine regulates
p53 activity and stability
Abstract :
- Post-translational addition of O-linked N-acetylglucosamine (O-GlcNAc) to p53 is known to occur, but the site of O-GlcNAcylation and its effects on p53 are not understood
- Here, we show that Ser 149 of p53 is O-GlcNAcylated and that this modification is associated with decreased phosphorylation of p53 at Thr 155 , which is a site that is targeted by the COP9 signalosome signalosome , resulting in decreased p53 ubiquitination
- Accordingly, O-GlcNAcylation at Ser 149 stabilizes p53 by blocking ubiquitin-dependent proteolysis
- Our results indicate that the dynamic interplay between O-GlcNAc and O-phosphate modifications coordinately regulate p53 stability and activity
Output (sent_index, trigger,
protein,
sugar,
site):
- 3. Ser, , -, O-GlcNAcylation, Ser 149
Output(Part-Of) (sent_index,
protein,
site):
- 1. p53, site
- 2. p53, Ser 149
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 3. p53, O-GlcNAcylation, Ser 149