Title : Crystal structure of the human
carboxypeptidase N (
kininase I ) catalytic
domain
Abstract :
- Human carboxypeptidase N ( CPN ), a member of the CPN /E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues
- Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa ( CPN2 ) domains each flanked by a 48 to 55 kDa catalytic ( CPN1 ) domain
- We have prepared and crystallized the recombinant C-terminally truncated catalytic domain of human CPN1 , and have determined and refined its 2.1 A crystal structure
- The structural analysis reveals that CPN1 has a pear-like shape, consisting of a 319 residue N-terminal catalytic domain and an abutting, cylindrically shaped 79 residue C-terminal beta-sandwich transthyretin ( TT ) domain , more resembling CPD-2 than CPM
- Like these other CPN /E members, two surface loops surrounding the active-site groove restrict access to the catalytic center, offering an explanation for why some larger protein carboxypeptidase inhibitors do not inhibit CPN
- Modeling of the Pro-Phe-Arg C-terminal end of the natural substrate bradykinin into the active site shows that the S1' pocket of CPN1 might better accommodate P1'- Lys than Arg residues , in agreement with CPN 's preference for cleaving off C-terminal Lys residues
- Three Thr residues at the distal TT edge of CPN1 are O-linked to N-acetyl glucosamine sugars; equivalent sites in the membrane-anchored CPM are occupied by basic residues probably involved in membrane interaction
- In tetrameric CPN , each CPN1 subunit might interact with the central leucine-rich repeat tandem of the cognate CPN2 subunit via a unique hydrophobic surface patch wrapping around the catalytic domain- TT interface, exposing the two active centers
Output (sent_index, trigger,
protein,
sugar,
site):
- 1. glycoprotein, , CPN, -, -
- 1. glycoprotein, , glycoprotein, -, -
- 7. Thr, , -, O-linked to N-acetyl glucosamine sugars, Thr residues
- 7. occupied, , -, -, sites
- 8. leucine-rich, , -, the central leucine-rich repeat tandem, leucine
- 8. subunit, , CPN2 subunit, the central leucine-rich repeat tandem, -
Output(Part-Of) (sent_index,
protein,
site):
- 0. carboxypeptidase N, domain
- 0. kininase I, domain
- 2. CPN1, domain
- 3. CPN1, domain
- 4. TT, domain
- 4. transthyretin, domain
- 7. CPM, sites
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):