PMID: 1823160

 

    Legend: Gene, Sites

Title : A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator

Abstract :

1. We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator ( mt-PA ), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (r mt-PA )
2. These studies have confirmed the published structures for rt-PA, but are not in agreement with some of the structures reported for mt-PA
3. In the latter glycoprotein we have identified a novel structure as the major oligosaccharide attached to Asn-184 and Asn-448
4. This is a biantennary oligosaccharide consisting of a fucosylated trimannosyl core to which are attached two GalNAc(1----4)GlcNAc antennae, one of which carries a sialic acid linked at the 6- position of the GalNAc
5. Minor constituents are sialylated on both or neither antennae
6. The sialylated GalNAc moiety is unique in N-linked glycoproteins
7. The majority of complex structures in r mt-PA contain N-acetyllactosamine moieties at both the Asn-184 and Asn-448 sites with the novel oligosaccharide occurring as a minor component at the Asn-184 site
8. This study demonstrates the power of mass spectrometric strategies based on high-field two-sector FAB-MS for structure elucidations of natural and recombinant glycoproteins

Output (sent_index, trigger, protein, sugar, site):

• 0. sialylated, , -, A novel sialylated N-acetylgalactosamine-containing oligosaccharide, -
• 0. sialylated, , -, the major complex-type structure, -
• 1. activator, , tissue plasminogen activator, the N-linked oligosaccharides, -
• 3. attached, , -, the major oligosaccharide, Asn-184 and Asn-448
• 3. glycoprotein, , glycoprotein, -, -
• 4. linked, , -, a sialic acid, position
• 6. glycoproteins, , glycoproteins, The sialylated GalNAc moiety, -
• 6. sialylated, , -, The sialylated GalNAc moiety, -
• 7. contain, , -, N-acetyllactosamine moieties, Asn-184 and Asn-448 sites
• 7. sites, , -, the novel oligosaccharide, Asn-184 and Asn-448 sites
• 7. structures, , mt-PA, structures, -
• 8. glycoproteins, , glycoproteins, -, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 3. tissue plasminogen activator, the major oligosaccharide, Asn-184 and Asn-448
• 7. mt-PA, N-acetyllactosamine moieties, Asn-184 and Asn-448 sites
• 7. mt-PA, the novel oligosaccharide, Asn-184 and Asn-448 sites

 

Protein	NCBI ID	SENTENCE INDEX