Title : Simultaneous glycosylation analysis of human serum
glycoproteins by high-performance liquid chromatography/tandem mass spectrometry
Abstract :
- Changes in the glycosylation of some serum proteins are associated with certain diseases
- In this study, we performed simultaneous site-specific glycosylation analysis of abundant serum glycoproteins by LC/Qq-TOF MS of human serum tryptic digest, the albumin of which was depleted
- The glycopeptide peaks on the chromatogram were basically assigned by database searching with modified peak-list text files of MS/MS spectra and then based on mass differences of glycan units from characterized glycopeptides
- Glycopeptide of IgG , haptoglobin and ceruloplasmin were confirmed by means of a comparison of their retention times and m/z values with those obtained by LC/MS of commercially available glycoproteins
- Mass spectrometric carbohydrate heterogeneity in the assigned glycopeptides was analyzed by an additional LC/MS
- We successfully demonstrated site-specific glycosylation of 23 sites in abundant serum glycoproteins
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycoproteins, , glycoproteins, -, -
- 0. glycosylation, , glycoproteins, -, -
- 1. glycosylation, , proteins, -, -
- 2. glycoproteins, , glycoproteins, -, -
- 2. glycosylation, , glycoproteins, -, -
- 3. glycopeptide, , -, -, glycopeptide
- 3. glycopeptides, , -, glycan units, glycopeptides
- 4. glycoproteins, , glycoproteins, -, -
- 5. glycopeptides, , -, -, glycopeptides
- 5. heterogeneity, , -, the assigned glycopeptides, glycopeptides
- 6. glycoproteins, , glycoproteins, -, -
- 6. glycosylation, , glycoproteins, -, sites
Output(Part-Of) (sent_index,
protein,
site):
- 4. IgG, Glycopeptide
- 4. ceruloplasmin, Glycopeptide
- 4. haptoglobin, Glycopeptide
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):