Title :
SPINKL , a Kazal-type
serine protease inhibitor-like
protein purified from mouse seminal vesicle fluid, is able to inhibit sperm capacitation
Abstract :
- We report a secreted serine protease inhibitor Kazal-type-like ( SPINKL ) protein
- The SPINKL protein was purified from mouse seminal vesicle secretions through a series of steps, including ion-exchange chromatography on a diethylaminoethyl-Sephacel column, gel filtration on a Sephadex G-75 column, and ion-exchange HPLC on a Q strong anion exchange column
- Further analysis identified several SPINKL proteins with various N-linked carbohydrates
- The SPINKL protein has six conserved cysteine residues that are nearly identical to those of members of the SPINK protein family
- It was noted that the SPINKL protein showed no inhibitory activities against common serine proteases such as trypsin , chymotrypsin , subtilisin , or elastase
- Spinkl mRNA and SPINKL proteins were found to be primarily expressed in seminal vesicles
- Immunohistochemistry revealed that the SPINKL protein occurred in the luminal fluid and mucosal epithelium of the seminal vesicles and was regulated by testosterone
- The SPINKL protein was able to bind onto sperm and enhance sperm motility
- Also, it was able to suppress BSA-stimulated sperm capacitation and block sperm-oocyte interactions in vitro, suggesting that SPINKL may be a decapacitation factor
Output (sent_index, trigger,
protein,
sugar,
site):
- 3. proteins, , SPINKL proteins, various N-linked carbohydrates, -
Output(Part-Of) (sent_index,
protein,
site):
- 4. SPINKL protein, cysteine residues
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):