PMID: 1872845

 

    Legend: Gene, Sites

Title : Carbohydrate analysis of human von Willebrand factor with horseradish peroxidase-conjugated lectins

Abstract :

1. Human von Willebrand factor ( vWF ) immobilized on a polyvinylidene difluoride membrane was subjected to binding assay with a series of horseradish peroxidase-conjugated lectins
2. The protein was reactive with concanavalin A , Ricinus communis agglutinin 120 , wheat germ agglutinin and Ulex europaeus agglutinin I (UEA-I) but not with peanut agglutinin before sialidase treatment
3. These reactivities were consistent with the major oligosaccharide structure reported except for UEA-I
4. The reactivity with UEA-I was greatly decreased after digestion of the protein with either alpha-L-fucosidase or peptide-N-glycosidase F , but no significant decrease was observed after mild alkaline treatment or delipidation
5. vWF and UEA-I have been independently used as a good marker for human endothelial cells
6. Our results indicate that vWF itself contains UEA-I reactive sugar chains in its Asn-linked oligosaccharides

Output (sent_index, trigger, protein, sugar, site):

• 6. Asn-linked, , -, its Asn-linked oligosaccharides, Asn
• 6. contains, , vWF, UEA-I reactive sugar chains, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
Human von Willebrand factor	7450	1
von Willebrand factor	7450	0
vWF	7450	1,5,6