PMID: 1883960

 

    Legend: Gene, Sites

Title : Marked cell-type-specific differences in glycosylation of human interleukin-6

Abstract :
  1. Human interleukin-6 ( IL-6 ) secreted by cytokine- or endotoxin-induced fibroblasts, monocytes, keratinocytes, endometrial stromal cells, and endothelial cells, when analyzed under denaturing and reducing conditions, consists of a set of differentially modified phosphoglycoproteins of molecular mass in the range from 23 to 30 kD (a set of at least three O-glycosylated 23- to 25-kD species and a set of at least three N- and O-glycosylated 28- to 30-kD species)
  2. The 23- to 25-kD and 28- to 30-kD fibroblast-derived IL-6 species have been separately purified to homogeneity with the use of a combination of lectin and immunoaffinity chromatography
  3. Glycosidase digestion experiments on such purified preparations confirmed that almost all human fibroblast-derived IL-6 species were O-glycosylated; additionally, the 28- to 30-kD species were N-glycosylated
  4. Amino acid sequencing revealed that the major amino terminus in the fibroblast-derived 23- to 25-kD O-glycosylated IL-6 was at Ala28 whereas the major amino terminus in the 28- to 30-kD N- and O-glycosylated IL-6 was at Val30 , suggesting that targeting of newly synthesized IL-6 polypeptides into the two different processing pathways in fibroblasts may be keyed to differences in the signal peptide cleavage site
  5. Unexpectedly, IL-6 "constitutively" secreted by the Epstein-Barr virus (EBV)-infected human and primate ( tamarin ) B-cell lines designated sfBJAB and sfBT, respectively, consisted of a major apparently unglycosylated 21-kD species and a minor 25-kD N-glycosylated species
  6. (ABSTRACT TRUNCATED AT 250 WORDS
Output (sent_index, trigger, protein, sugar, site):
  • 0. glycosylation, , interleukin-6, -, -
  • 1. phosphoglycoproteins, , -, differentially modified phosphoglycoproteins, -
  • 3. O-glycosylated, , -, almost all human fibroblast-derived IL-6 species, -
  • 4. O-glycosylated, , IL-6, -, -
Output(Part-Of) (sent_index, protein, site):
  • 4. IL-6, polypeptides
  • 4. IL-6, terminus
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

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