PMID: 18952059

 

    Legend: Gene, Sites

Title : Putative sperm fusion protein IZUMO and the role of N-glycosylation

Abstract :

1. IZUMO is the mouse sperm protein proven to be essential for fusion with eggs
2. It contains one immunoglobulin-like domain with a conserved glycosylation site within
3. In the present paper, we produced transgenic mouse lines expressing unglycosylated IZUMO (N204Q -IZUMO ) in Izumo1 -/- background
4. The expression of N204Q -IZUMO rescued the infertile phenotype of IZUMO disrupted mice, indicating glycosylation is not essential for fusion-facilitating activity of IZUMO
5. The N204Q -IZUMO was produced in testis in comparable amounts to wild-type IZUMO , but the amount of N204Q -IZUMO on sperm was significantly decreased by the time sperm reached the cauda epididymis
6. These data suggest that glycosylation is not essential for the function of IZUMO , but has a role in protecting it from fragmentation in cauda epididymis

Output (sent_index, trigger, protein, sugar, site):

• 2. glycosylation, , -, -, site
• 3. unglycosylated, , -IZUMO, -, -
• 3. unglycosylated, , IZUMO, -, -
• 4. glycosylation, , IZUMO, -, -
• 6. glycosylation, , IZUMO, -, -

Output(Part-Of) (sent_index, protein, site):

• 2. -, site

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
Izumo1	73456	3
-IZUMO	73456	3,4,5
IZUMO	73456	0,1,3,4,5,6