Glyco

Title : Tissue plasminogen activator has an O-linked fucose attached to threonine-61 in the epidermal growth factor domain

Abstract :

An unusual type of glycosylation has been observed for tissue plasminogen activator ( t-PA )
The monosaccharide fucose is glycosidically linked to threonine-61 in the epidermal growth factor region of t-PA
The presence of O-linked fucose was demonstrated by carbohydrate analysis and mass spectrometry of tryptic and chymotryptic peptides that contain this site
The susceptibility of the fucose residue to alpha-fucosidase indicated that it was in the alpha-anomeric configuration
Fucosylation of threonine-61 was observed in t-PA isolated from the Bowes melanoma cell line and from recombinant expression systems using Chinese hamster ovary or human embryonic kidney cells
Fucosylation of the homologous residue in prourokinase has also been reported recently
Our results indicate that this novel type of glycosylation may be common to the epidermal growth factor domains found in coagulation and fibrinolytic proteins and, therefore, suggest that the modification may have functional significance

Output (sent_index, trigger, protein, sugar, site):

0. attached, , -, an O-linked fucose, threonine-61
0. has, , Tissue plasminogen activator, an O-linked fucose, -
2. linked, , -, The monosaccharide fucose, threonine-61
3. carbohydrate, , -, -, peptides
5. Fucosylation, , t-PA, -, threonine-61
5. observed, , t-PA, Fucosylation, -
5. threonine-61, , -, Fucosylation, threonine-61
6. Fucosylation, , prourokinase, Fucosylation, residue in
6. residue, , -, Fucosylation, residue in

Output(Part-Of) (sent_index, protein, site):

0. factor, domain
2. factor, region
2. t-PA, region
3. -, site
7. factor, domains
7. proteins, domains

*Output_Site_Fusion* (sent_index, protein, sugar, site):

0. Tissue plasminogen activator, an O-linked fucose, threonine-61
2. Tissue plasminogen activator, The monosaccharide fucose, threonine-61
5. t-PA, -, threonine-61
5. t-PA, Fucosylation, threonine-61