PMID: 19141282

 

    Legend: Gene, Sites

Title : Structural and biophysical studies of the human IL-7 / IL-7Ralpha complex

Abstract :

1. IL-7 and IL-7Ralpha bind the gamma(c) receptor , forming a complex crucial to several signaling cascades leading to the development and homeostasis of T and B cells
2. We report that the IL-7Ralpha ectodomain uses glycosylation to modulate its binding constants to IL-7 , unlike the other receptors in the gamma(c) family
3. IL-7 binds glycosylated IL-7Ralpha 300-fold more tightly than unglycosylated IL-7Ralpha , and the enhanced affinity is attributed primarily to an accelerated on rate
4. Structural comparison of IL-7 in complex to both forms of IL-7Ralpha reveals that glycosylation does not participate directly in the binding interface
5. The SCID mutations of IL-7Ralpha locate outside the binding interface with IL-7 , suggesting that the expressed mutations cause protein folding defects in IL-7Ralpha
6. The IL-7 / IL-7Ralpha structures provide a window into the molecular recognition events of the IL-7 signaling cascade and provide sites to target for designing new therapeutics to treat IL-7-related diseases

Output (sent_index, trigger, protein, sugar, site):

• 3. glycosylated, , IL-7Ralpha, -, -
• 3. unglycosylated, , IL-7Ralpha, -, -

Output(Part-Of) (sent_index, protein, site):

• 2. IL-7Ralpha, ectodomain

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
IL-7	3574	0,1,2,3,4,5,6
IL-7Ralpha	3575	0,1,2,3,4,5,6