PMID: 19153605

 

    Legend: Gene, Sites

Title : structure of the F-spondin domain of mindin , an integrin ligand and pattern recognition molecule

Abstract :

1. Mindin ( spondin-2 ) is an extracellular matrix protein of unknown structure that is required for efficient T-cell priming by dendritic cells
2. Additionally, mindin functions as a pattern recognition molecule for initiating innate immune responses
3. These dual functions are mediated by interactions with integrins and microbial pathogens, respectively
4. Mindin comprises an N-terminal F-spondin ( FS ) domain and C-terminal thrombospondin type 1 repeat (TSR)
5. We determined the structure of the FS domain at 1.8-A resolution
6. The structure revealed an eight-stranded antiparallel beta-sandwich motif resembling that of membrane-targeting C2 domains , including a bound calcium ion
7. We demonstrated that the FS domain mediates integrin binding and identified the binding site by mutagenesis
8. The mindin FS domain therefore represents a new integrin ligand
9. We further showed that mindin recognizes lipopolysaccharide (LPS) through its TSR domain , and obtained evidence that C-mannosylation of the TSR influences LPS binding
10. Through these dual interactions, the FS and TSR domains of mindin promote activation of both adaptive and innate immune responses

Output (sent_index, trigger, protein, sugar, site):

• 9. C-mannosylation, , -, the TSR, -

Output(Part-Of) (sent_index, protein, site):

• 0. mindin, domain
• 0. structure of the F-spondin, domain
• 10. FS, domains
• 10. mindin, domains
• 4. FS, domain
• 4. N-terminal F-spondin, domain
• 5. structure of the FS, domain
• 7. FS, domain
• 8. mindin FS, domain

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
mindin	10417	0,9,10
spondin-2	10417	1
Additionally, mindin	10417	2
N-terminal F-spondin	10418	4
mindin FS	10417	8
structure of the F-spondin	10418	0
Mindin	10417	1,4