PMID: 19664639

 

    Legend: Gene, Sites

Title : Crystal structure of the frizzled-like cysteine-rich domain of the receptor tyrosine kinase MuSK

Abstract :

1. Muscle-specific kinase ( MuSK ) is an essential receptor tyrosine kinase for the establishment and maintenance of the neuromuscular junction (NMJ)
2. Activation of MuSK by agrin , a neuronally derived heparan-sulfate proteoglycan, and LRP4 ( low-density lipoprotein receptor-related protein-4 ), the agrin receptor , leads to clustering of acetylcholine receptors on the postsynaptic side of the NMJ
3. The ectodomain of MuSK comprises three immunoglobulin-like domains and a cysteine-rich domain (Fz-CRD) related to those in Frizzled proteins , the receptors for Wnts
4. Here, we report the crystal structure of the MuSK Fz-CRD at 2.1 A resolution
5. The structure reveals a five-disulfide-bridged domain similar to CRDs of Frizzled proteins but with a divergent C-terminal region
6. An asymmetric dimer present in the crystal structure implicates surface hydrophobic residues that may function in homotypic or heterotypic interactions to mediate co-clustering of MuSK , rapsyn , and acetylcholine receptors at the NMJ

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

• 0. MuSK, domain
• 3. MuSK, ectodomain

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
MuSK	4593	1,2,3,6
LRP4	4038	2
structure of the MuSK	4593	4
agrin	375790	2
agrin receptor	375790	2
Muscle-specific kinase	4593	1
rapsyn	5913	6