PMID: 20043878

 

    Legend: Gene, Sites

Title : Identification of a new secretory factor , CCDC3 / Favine , in adipocytes and endothelial cells

Abstract :

1. The vascular system secretes many bioactive factors
2. In a gene chip database , we searched for novel genes with signal sequences that are specifically expressed in murine aorta, and focused on one gene previously named CCDC3 (NCBI nucleotide entry NM_028804), and we designated as Favine ( fat/vessel-derived secretory protein )
3. Northern blot analysis revealed that CCDC3 was expressed abundantly in the aorta and adipose tissues
4. The mRNA levels of CCDC3 were higher in adipose tissues of obese db/db mice than control mice, and induced during differentiation of rat primary adipocytes
5. In differentiated adipocytes, CCDC3 mRNA expression was enhanced by insulin and pioglitazone, a PPARgamma agonist, and suppressed by TNF-alpha , isoproterenol and norepinephrine
6. Transient expression experiments followed by N-terminal amino acid sequence analysis revealed secretion of CCDC3 protein into the culture medium, which was dose-dependently reduced by brefeldin A , an inhibitor of Golgi-mediated secretory pathway
7. When expressed in COS-7 cells, CCDC3 protein was post-transcriptionally modified with N-glycosylation, and formed a dimer complex
8. These results indicate that CCDC3 is a protein secreted by adipocytes and endothelial cells, and that its level is regulated both hormonally and nutritionally

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
PPARgamma	19016	5
TNF-alpha	21926	5
CCDC3 protein	74186	6,7
Favine	74186	0,2
CCDC3	74186	0,2,3,4,5,8