PMID: 2013294

 

    Legend: Gene, Sites

Title : Primary structure of a new actin-binding protein from human seminal plasma

Abstract :

1. Secretory actin-binding protein ( SABP ), a glycoprotein from human seminal plasma, was isolated according to Akiyama and Kimura [Akiyama, K. & Kimura, H. (1990) Biochim
2. Biophys
3. Acta 1040, 206-210]
4. The complete amino acid sequence of SABP was determined with the aid of fragments generated by trypsin, Staphylococcus aureus V8 protease and pepsin
5. The single polypeptide chain of SABP contains 118 amino acids with a calculated Mr of 13,506 and pyroglutamic acid as the N-terminal residue
6. A single N-glycosidic carbohydrate moiety is located at Asn77
7. The carbohydrate composition shows an unusually high amount of fucose
8. The arrangement of the two disulfide bonds is Cys37-Cys63 and Cys61-Cys95
9. Sequence comparison revealed a high degree of similarity with a 14-kDa submandibular gland protein from mouse (45% identity and 64% similarity)
10. SABP is identical with a prolactin-inducible protein and a protein termed gross cystic disease fluid protein 15 ( sequences translated from cDNA clones), both from human breast tissues
11. Although SABP was also detected in saliva, in extracts of the submandibular gland and seminal vesicles, little is known of its function

Output (sent_index, trigger, protein, sugar, site):

• 1. glycoprotein, , Secretory actin-binding protein, -, -
• 1. glycoprotein, , glycoprotein, -, -
• 6. Asn77, , -, A single N-glycosidic carbohydrate moiety, Asn77
• 6. located, , -, A single N-glycosidic carbohydrate moiety, Asn77

Output(Part-Of) (sent_index, protein, site):

• 4. SABP, sequence

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 6. Secretory actin-binding protein, A single N-glycosidic carbohydrate moiety, Asn77

 

Protein	NCBI ID	SENTENCE INDEX
SABP	5304	1,4,5,10,11
14-kDa submandibular gland protein	18716	9
gross cystic disease fluid protein 15	5304	10
Secretory actin-binding protein	5304	1