PMID: 2023947

 

    Legend: Gene, Sites

Title : Characterization of a posttranslational fucosylation in the growth factor domain of urinary plasminogen activator

Abstract :

1. A posttranslational modification site in natural and recombinant urinary-type plasminogen activators (urokinases; EC 3.4.21.31) has been localized to Thr-18 , in the growth factor domain of the molecule
2. This is the region of urinary plasminogen activator responsible for its specific receptor binding
3. An unusual carbohydrate-protein linkage, a single monosaccharide, fucose, covalently attached directly to threonine in the peptide , is described here
4. The glycan moiety and the site of modification have been identified with mass spectrometry and confirmed by carbohydrate com position analysis, Edman degradation, and one- and two-dimensional NMR studies
5. This type of modification is normally not detected without mass spectrometry because the fucose- threonine bond is hydrolyzed under standard acidic conditions of the amino acid analysis and Edman sequencing
6. This modification may be widely found in other proteins

Output (sent_index, trigger, protein, sugar, site):

• 0. fucosylation, , -, the growth factor domain, domain
• 3. attached, , -, An unusual carbohydrate-protein linkage, peptide
• 3. attached, , -, An unusual carbohydrate-protein linkage, threonine
• 3. attached, , -, a single monosaccharide, peptide
• 3. attached, , -, a single monosaccharide, threonine
• 3. attached, , -, fucose, peptide
• 3. attached, , -, fucose, threonine
• 5. fucose-threonine, , -, the fucose-threonine bond, threonine

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX