PMID: 21138434

 

    Legend: Gene, Sites

Title : The identification of N-glycosylated residues of the human 5-HT3B receptor subunit : importance for cell membrane expression

Abstract :
  1. The 5-hydroxytryptamine 3 ( 5-HT(3 )) receptor is a pentameric ligand-gated ion channel with potential molecular isoforms arising from different subunit combinations and/or different post-translational modifications of the individual subunits
  2. Since N-glycosylation of the 5-HT3A subunit impacts cell surface trafficking, the presence of N-glycosylation of the human (h) 5-HT3B subunit and the influence upon cell membrane expression was investigated
  3. Following transient expression of the h5-HT3B subunit by human embryonic kidney cells (HEK293 cells) stably expressing the h5-HT3A subunit , the N-glycosylation inhibitor tunicamycin reduced the size of the predominant h5-HT3B-immunoreactive protein (∼ 55 kDa reduced to ∼ 40 kDa)
  4. Disruption of each consensus N-glycosylation sequences in the h5-HT3B subunit (N31S, N75S, N117S, N147S and N182S) resulted in a reduced molecular weight (by ∼ 2-4 kDa) of each mutant when expressed by HEK293 cells stably expressing the h5-HT3A subunit
  5. Immunocytochemical studies demonstrated that disruption of each of the N-glycosylation sequences (individually or combined) reduced the expression of the mutant h5-HT3B subunit protein in the cell membrane when co-expressed with the h5-HT3A subunit
  6. The present study has identified utilised N-glycosylation sites of the h5-HT3B subunit and demonstrated that they promote subunit expression in the cell membrane; a prerequisite for 5-HT(3 ) receptor function
Output (sent_index, trigger, protein, sugar, site):
  • 0. N-glycosylated, , subunit, -, residues
  • 2. N-glycosylation, , 5-HT3A subunit, -, -
  • 2. N-glycosylation, , 5-HT3B subunit, -, -
  • 6. N-glycosylation, , h5-HT3B subunit, -, sites
  • 6. identified, , -, -, prerequisite
Output(Part-Of) (sent_index, protein, site):
  • 0. subunit, residues
  • 4. h5-HT3B subunit, sequences
  • 6. h5-HT3B subunit, sites
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
5-HT3B receptor 9177 0
5-HT3A subunit 3359 2
5-HT3B subunit 9177 2