Title : Pregnancy-associated changes in oligomannose oligosaccharides of human and bovine
uromodulin (
Tamm-Horsfall glycoprotein )
Abstract :
- The urinary glycoprotein uromodulin ( Tamm-Horsfall glycoprotein ) exhibits a pregnancy-associated ability to inhibit antigen-specific T cell proliferation, and the activity is associated with a carbohydrate moiety [Muchmore and Decker (1985) Science 229:479-81; Hession et al., (1987) Science 237:1479-84; Muchmore, Shifrin and Decker (1987) J Immunol 138:2547-53]
- We report here that the Man6(7)GlcNAc2-R glycopeptides derived from uromodulin inhibit antigen-specific T cell proliferation by 50% at 0.2-2 microM, and further studies, reported elsewhere, confirm that oligomannose glycopeptides from other sources are also inhibitory, with Man9GlcNAc2-R the most inhibitory of those tested [Muchmore et al., J Leukocyte Biol (in press)]
- In this work, we have extended the observation of pregnancy-associated inhibitory activity to a second species, and have compared the oligomannose profile of Tamm-Horsfall glycoprotein (nonpregnant) with that of uromodulin (pregnant) derived from both human and bovine sources
- Surprisingly, there was a pregnancy-associated decrease in the total content of oligomannose chains due predominantly to a reduction in Man5GlcNAc2-R and Man6GlcNAc2-R
- Man7GlcNAc2-R, which did not decrease with pregnancy, comprised a significantly greater proportion of the total oligomannose chains in pregnant vs. nonpregnant samples from both species (human; 34.6% vs. 25.9%: bovine; 14.4% vs. 7.2%)
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycoprotein, , Tamm-Horsfall glycoprotein, -, -
- 0. glycoprotein, , uromodulin, -, -
- 0. uromodulin, , uromodulin, oligomannose oligosaccharides, -
- 1. glycoprotein, , Tamm-Horsfall glycoprotein, -, -
- 1. glycoprotein, , glycoprotein, -, -
- 1. glycoprotein, , uromodulin, -, -
- 2. derived, , uromodulin, the Man6(7)GlcNAc2-R glycopeptides, -
- 2. glycopeptides, , -, -, glycopeptides
- 3. glycoprotein, , Tamm-Horsfall glycoprotein, -, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):