PMID: 21422170

 

    Legend: Gene, Sites

Title : Structure of the human activating natural cytotoxicity receptor NKp30 bound to its tumor cell ligand B7-H6

Abstract :

1. Natural killer (NK) cells are lymphocytes of the in na te i mmune syste m that participate in the eli m ination of tumor cells
2. In humans, the activating natural cytotoxicity receptors ( NCRs ) NKp30 , NKp44 , and NKp46 play a major role in NK cell-mediated tumor cell lysis
3. NKp30 recognizes B7-H6 , a member of the B7 family which is expressed on tumor, but not healthy, cells
4. To understand the basis for tumor surveillance by NCRs , we determined the structure of NKp30 , a member of the CD28 family which includes CTLA-4 and PD-1 , in complex with B7-H6
5. The overall organization of the NKp30- B7-H6-activating complex differs considerably fro m those of the CTLA-4-B7 and PD-1- PD-L T cell inhibitory complexes
6. Whereas CTLA-4 and PD-1 use only the front β-sheet of their Ig-like domain to bind ligands, NKp30 uses both front and back β-sheets, resulting in engagement of B7-H6 via the side, as well as face , of the β-sandwich
7. Moreover, B7-H6 contacts NKp30 through the complementarity-determining region ( CDR )-like loops of its V-like domain in an antibody-like interaction that is not observed for B7 or PD-L
8. This first structure of an NCR bound to ligand provides a template for designing molecules to stimulate NKp30-mediated cytolytic activity for tumor immunotherapy

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
B7-H6	374383	3,4,5,6
PD-1	5133	4,6
i	374383	1
na	259197	1
NCR	4827	8
NKp30	259197	0,2,3,5,6,7,8
m	940	1
NKp46	9437	2
NKp44	9436	2
PD-L	5133	7
Moreover, B7-H6	374383	7
structure of NKp30	259197	4