PMID: 21569239

 

    Legend: Gene, Sites

Title : The structure of the Ca²+-binding, glycosylated F-spondin domain of F-spondin - A C2-domain variant in an extracellular matrix protein

Abstract :
  1. BACKGROUND: F-spondin is a multi-domain extracellular matrix ( ECM ) protein and a contact-repellent molecule that directs axon outgrowth and cell migration during development
  2. The reelin_N domain and the F-spondin domain (FS domain ) comprise a proteolytic fragment that interacts with the cell membrane and guides the projection of commissural axons to floor plate
  3. The FS domain is found in F-spondins, mindins, M-spondin and amphiF-spondin
  4. RESULTS: We present the crystal structure of human F-spondin FS domain at 1.95Å resolution
  5. The structure reveals a Ca2 +-binding C2 domain variant with an 8-stranded antiparallel β-sandwich fold
  6. Though the primary sequences of the FS domains of F-spondin and mindin are less than 36% identical, their overall structures are very similar
  7. The unique feature of F-spondin FS domain is the presence of three disulfide bonds associated with the N- and C-termini of the domain and a highly conserved N-linked glycosylation site
  8. The integrin-binding motif found in mindin is not conserved in the F-spondin FS domain
  9. CONCLUSION: The structure of the F-spondin FS domain completes the structural studies of the multiple-domain ECM molecule
  10. The homology of its core structure to a common Ca2 +- and lipid-binding C2 domain suggests that the F-spondin FS domain may be responsible for part of the membrane targeting of F-spondin in its regulation of axon development
  11. The structural properties of the FS domain revealed in this study pave the way for further exploration into the functions of F-spondin
Output (sent_index, trigger, protein, sugar, site):
  • 0. glycosylated, , F-spondin, -, domain
  • 7. glycosylation, , -, -, site
Output(Part-Of) (sent_index, protein, site):
  • 0. F-spondin, domain
  • 10. F-spondin, domain
  • 2. F-spondin, domain
  • 3. M-spondin, domain
  • 3. amphiF-spondin, domain
  • 4. F-spondin, domain
  • 6. F-spondin, domains
  • 6. mindin, domains
  • 7. F-spondin, domain
  • 8. F-spondin, domain
  • 8. mindin, motif
  • 9. structure of the F-spondin, domain
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
structure of the F-spondin 10418 9
M-spondin 10417 3
mindin 10417 6,8
F-spondin 10418 0,1,2,4,6,7,8,10,11