PMID: 2243102

 

    Legend: Gene, Sites

Title : The polylactosaminoglycans of human lysosomal membrane glycoproteins lamp-1 and lamp-2

Abstract :
  1. Localization on the peptide backbones
  2. Lysosome membrane glycoproteins , lamp-1 and lamp-2 , have been shown to contain 18 and 16 N-glycans, some of which are modified by poly-N-acetyl-lactosamine
  3. We have localized the polylactosaminoglycans to specific sites on lamp-1 and lamp-2 purified from human chronic myelogenous leukemia cells
  4. Polylactosaminoglycan-containing glycopeptides, obtained by trypsin, pepsin, and V8 protease digestion of the glycoproteins , were isolated by Datura stramonium agglutinin affinity chromatography, gel filtration, and reverse phase high performance liquid chromatography
  5. The poly-N-acetyllactosaminyl structures of isolated glycopeptides were confirmed by the susceptibility of their released oligosaccharides to endo- beta-galactosidase
  6. Amino acid analysis and sequencing demonstrated that polylactosaminoglycans were located at Asn-34, Asn-93 and/or Asn-102, and Asn-195 and/or Asn-200 in lamp-1 , and at Asn-4 and/or Asn-10, and Asn-279 in lamp-2
  7. These results indicated that only certain glycosylation sites can be selectively modified by poly-N-acetyllactosamine, and those sites may confer the requirement by beta 1---- 3-N-acetylglucosaminyl transferase
Output (sent_index, trigger, protein, sugar, site):
  • 0. glycoproteins, , glycoproteins, The polylactosaminoglycans, -
  • 2. contain, , glycoproteins, 18 and 16 N-glycans, -
  • 2. contain, , lamp-1, 18 and 16 N-glycans, -
  • 2. contain, , lamp-2, 18 and 16 N-glycans, -
  • 2. glycoproteins, , glycoproteins, -, -
  • 2. glycoproteins, , lamp-1, -, -
  • 2. glycoproteins, , lamp-2, -, -
  • 3. polylactosaminoglycans, , lamp-1, polylactosaminoglycans, -
  • 3. polylactosaminoglycans, , lamp-2, polylactosaminoglycans, -
  • 4. glycoproteins, , glycoproteins, -, -
  • 5. glycopeptides, , -, -, glycopeptides
  • 6. located, , lamp-2, polylactosaminoglycans, Asn-34, Asn-93
  • 7. glycosylation, , -, -, sites
  • 7. modified, , -, poly-N-acetyllactosamine, sites
Output(Part-Of) (sent_index, protein, site):
  • 6. lamp-1, Asn-34, Asn-93
*Output_Site_Fusion* (sent_index, protein, sugar, site):
  • 6. lamp-2, polylactosaminoglycans, Asn-34, Asn-93

 

 

Protein NCBI ID SENTENCE INDEX
lamp-2 3920 0,2,3,6
lamp-1 3916 0,2,3,6
beta-galactosidase 2720 5