PMID: 22448645

 

    Legend: Gene, Sites

Title : Biochemical and pharmacological study of N-linked glycosylation of the human serotonin 5-HT₇a receptor

Abstract :

1. The 5-hydroxytryptamine (5-HT)(7 (a)) receptor is a G-protein-coupled receptor critically involved in human psychiatric and neurological disorders
2. In the present study, we evaluate the presence and the functional role of N-glycosylation of the human 5-HT(7 ) receptor
3. Western blot analysis of HEK293T cells transiently expressing the 5-HT(7 (a)) receptor in the presence of tunicamycin gave rise to a band shift, indicating the existence of an N-glycosylated form of the 5-HT(7 (a)) receptor
4. To further investigate this, we mutated the two predicted N-glycosylation sites (N5Q and N66Q) and compared the molecular mass of the immunoreactive bands with those of the wild-type receptor , indicating that both asparagines were N-glycosylated
5. The mutant receptors had the same binding affinity for [(3) H]5-CT and the same potency and efficacy with regard to 5-HT-induced activation of adenylyl cyclase
6. However, there was a reduction in maximal ligand binding for the single and double mutants compared to the wild-type receptor
7. Next, membrane labelling and immunocytochemical studies demonstrated that the N-glycosylation mutants were expressed at the cell surface
8. We conclude that N-glycosylation is not important for cell surface expression of the 5-HT(7 ) receptor

Output (sent_index, trigger, protein, sugar, site):

• 0. glycosylation, , 5-HT₇a, -, -
• 3. N-glycosylated, , N-glycosylated form of the 5-HT(7, -, -
• 3. N-glycosylated, , receptor, -, -
• 4. N-glycosylated, , -, -, asparagines
• 4. N-glycosylation, , -, -, sites
• 8. N-glycosylation, , receptor, -, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX