Glyco

Title : Human genetically polymorphic deoxyribonuclease : purification, characterization, and multiplicity of urine deoxyribonuclease I

Abstract :

A deoxyribonuclease I was purified from the urine of a 46-year-old male (a single individual) by using a series of column chromatographies to a homogeneous state as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis
The enzyme was found to be a glycoprotein , containing 1 fucose, 7 galactose, 10 mannose, 6 glucosamine, and 2 sialic acid residues per molecule
The N-terminal amino acid sequence up to the 27th residue of the enzyme was similar to that of pancreatic deoxyribonuclease I from bovine and other species
The catalytic properties of the enzyme derived from a single individual closely resembled those of deoxyribonuclease I purified from human urine collected from several volunteers [Ito, K. et al. (1984) J. Biochem
95, 1399-1406]
The purified enzyme was found to consist of multiple forms with different pI values
These findings are compatible with the existence of genetic polymorphism of deoxyribonuclease I in human urine previously reported [Kishi, K. et al. (1989) Hum
Genet
81, 295-297]
This multiplicity of the urine enzyme might be due to variations in the primary structure and/or differences in the content of sialic acid

Output (sent_index, trigger, protein, sugar, site):

2. containing, , enzyme, 1 fucose, -
2. containing, , enzyme, 10 mannose, -
2. containing, , enzyme, 2 sialic acid residues, -
2. containing, , enzyme, 6 glucosamine, -
2. containing, , enzyme, 7 galactose, -
2. containing, , glycoprotein, 1 fucose, -
2. containing, , glycoprotein, 10 mannose, -
2. containing, , glycoprotein, 2 sialic acid residues, -
2. containing, , glycoprotein, 6 glucosamine, -
2. containing, , glycoprotein, 7 galactose, -
2. glycoprotein, , enzyme, -, -
2. glycoprotein, , glycoprotein, -, -

Output(Part-Of) (sent_index, protein, site):

3. enzyme, residue

*Output_Site_Fusion* (sent_index, protein, sugar, site):