Title : Determination of
cathepsin V activity and intracellular trafficking by N-glycosylation
Abstract :
- Cathepsin V ( L2 ), a lysosomal cysteine protease , is a member of cathepsin family, relating to cancer invasion and metastasis
- Cathepsin V contains two predicted N-glycosylation sites , but it has not been reported whether cathepsin V is glycosylated or not
- In this study, we clarified the role of N-glycosylation of cathepsin V for its functions
- We demonstrated that cathepsin V is N-glycosylated at both Asn(221) and Asn(292) using mass spectrometry and site-directed mutagenesis
- N-glycosylation of cathepsin V was important for transportation to lysosome, secretion, and activity in HT1080 cells
- These data demonstrated that functions of cathepsin V are controlled by N-glycosylation
Output (sent_index, trigger,
protein,
sugar,
site):
- 2. N-glycosylation, , -, -, sites
- 2. glycosylated, , cathepsin V, -, -
- 3. N-glycosylation, , cathepsin V, -, -
- 4. N-glycosylated, , -, -, Asn(221) and Asn(292)
- 4. N-glycosylated, , cathepsin V, -, Asn(221) and Asn(292)
- 5. N-glycosylation, , cathepsin V, -, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 4. cathepsin V, -, Asn(221) and Asn(292)