PMID: 23139753

 

    Legend: Gene, Sites

Title : Human epididymis protein-4 ( HE-4 ): a novel cross-class protease inhibitor

Abstract :

1. Epididymal proteins represent the factors necessary for maturation of sperm and play a crucial role in sperm maturation
2. HE-4 , an epididymal protein , is a member of whey acidic protein four-disulfide core ( WFDC ) family with no known function
3. A WFDC protein has a conserved WFDC domain of 50 amino acids with eight conserved cystine residue
4. HE-4 is a 124 amino acid long polypeptide with two WFDC domains
5. Here, we show that HE-4 is secreted in the human seminal fluid as a disulfide-bonded homo-trimer and is a cross-class protease inhibitor inhibits some of the serine , aspartyl and cysteine proteases tested using hemoglobin as a substrate
6. Using SPR we have also observed that HE-4 shows a significant binding with all these proteases
7. Disulfide linkages are essential for this activity
8. Moreover, HE-4 is N-glycosylated and highly stable on a wide range of pH and temperature
9. Taken together this suggests that HE-4 is a cross-class protease inhibitor which might confer protection against microbial virulence factors of proteolytic nature

Output (sent_index, trigger, protein, sugar, site):

• 8. N-glycosylated, , Moreover, HE-4, -, -

Output(Part-Of) (sent_index, protein, site):

• 3. WFDC, domain
• 3. protein, domain
• 4. WFDC, domains

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX