PMID: 23756651

 

    Legend: Gene, Sites

Title : The structural basis of R-spondin recognition by LGR5 and RNF43

Abstract :

1. R-spondins (RSPOs) enhance Wnt signaling, affect stem cell behavior, bind to leucine-rich repeat-containing G-protein-coupled receptors 4-6, ( LGR4-6 ) and the transmembrane E3 ubiquitin ligases RING finger 43/zinc and RING finger 3 (RNF43 / ZNRF3 )
2. The structure of RSPO1 bound to both LGR5 and RNF43 ectodomains confirms their physical linkage
3. RSPO1 is sandwiched by LGR5 and RNF43 , with its rod module of the cysteine-rich domain (CRD) contacting LGR5 and a hairpin inserted into RNF43
4. LGR5 does not contact RNF43 but increases the affinity of RSPO1 to RNF43 , supporting LGR5 as an engagement receptor and RNF43 as an effector receptor
5. Disease mutations map to the RSPO1- RNF43 interface, which promises therapeutic targeting

Output (sent_index, trigger, protein, sugar, site):

• 1. leucine-rich, , -, leucine-rich repeat-containing G-protein-coupled receptors 4-6, leucine

Output(Part-Of) (sent_index, protein, site):

• 2. RNF43, ectodomains

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
LGR5	8549	0,2,3,4
RSPO1	284654	3,4,5
ZNRF3	84133	1
RNF43	54894	0,2,3,4,5
3 (RNF43	54894	1
LGR4-6	55366	1
structure of RSPO1	284654	2