Title : Allelic variation of
killer cell immunoglobulin-like receptor 2DS5 impacts glycosylation altering cell surface expression levels
Abstract :
- Natural killer cell stimulatory receptor gene, KIR2DS5 , is polymorphic
- While KIR2DS5 *002 is most frequently observed, other alleles have also been found
- The proteins encoded by these alleles ( KIR2DS5 *002-*009) are expressed at varying levels on the surface of NKL and Jurkat transfectants
- Gel electrophoresis of all allelic products showed two isoforms which differ in the extent of maturation of N-linked glycosylation
- These isoforms differed in intensity and molecular weight among the allelic products
- Site-directed mutagenesis was used to identify polymorphic variation at residues 123 and 157 as key in altering glycosylation and levels of surface expression
Output (sent_index, trigger,
protein,
sugar,
site):
- 3. KIR2DS5, , KIR2DS5, these alleles, -
Output(Part-Of) (sent_index,
protein,
site):
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):