Title : β
-catenin is O-GlcNAc glycosylated at
Serine 23 : implications for β
-catenin 's subcellular localization and transactivator function
Abstract :
- BACKGROUND: We have previously reported that β -catenin is post-translationally modified with a single O-linked attachment of β-N-acetyl-glucosamine (O-GlcNAc)
- We showed that O-GlcNAc regulated β -catenin 's subcellular localization and transcriptional activity
- OBJECTIVE: The objectives of this investigation were to identify the putative O-GlcNAc sites of β -catenin and the relevance of identified sites in the regulation of β -catenin 's localization and transcriptional activity
- METHOD: Missense mutations were introduced to potential O-GlcNAc sites of pEGFP-C2-N-Terminal- or pEGFP-C2-Wild Type-β -catenin by site-directed mutagenesis
- We determined the levels of O-GlcNAc-β-catenin, subcellular localization, interaction with binding partners and transcriptional activity of the various constructs
- RESULTS: Serine 23 of β -catenin was determined as a site for O-GlcNAc modification which regulated its subcellular distribution, its interactions with cellular partners and consequently its transcriptional activity
- SIGNIFICANCE: O-GlcNAcylation of Serine 23 is a novel regulatory modification for β -catenin 's subcellular localization and transcriptional activity
- This study is the first report to characterize site specific regulation of β -catenin by the O-GlcNAc modification
Output (sent_index, trigger,
protein,
sugar,
site):
- 0. glycosylated, , -catenin, -, Serine 23
- 3. sites, , -, the putative O-GlcNAc sites, sites
- 3. β-catenin, , -catenin, the putative O-GlcNAc sites, -
- 4. Type-β-catenin, , -catenin, potential O-GlcNAc sites, -
- 7. Serine, , -, O-GlcNAcylation, Serine 23
Output(Part-Of) (sent_index,
protein,
site):
- 3. -catenin, sites
- 6. -catenin, Serine 23
- 6. -catenin, site
*Output_Site_Fusion* (sent_index,
protein,
sugar,
site):
- 0. -catenin, -, Serine 23
- 7. -catenin, O-GlcNAcylation, Serine 23