PMID: 24365146

 

    Legend: Gene, Sites

Title : N-Linked glycosylation of the superoxide-producing NADPH oxidase Nox1

Abstract :

1. Nox1 is a membrane-integrated protein that belongs to the Nox family of superoxide-producing NADPH oxidases
2. Here we show that human Nox1 undergoes glycosylation at Asn-162 and Asn-236 in the second and third extracellular loops, respectively
3. Simultaneous threonine substitution for these residues completely abrogates the glycosylation, but does not prevent Nox1 from forming a heterodimer with p22 (phox), trafficking to the cell surface, or producing superoxide
4. In the absence of p22 (phox), Nox1 is transported to the plasma membrane mainly as a form with high mannose N-glycans, although their conversion into complex N-glycans is induced by expression of p22 (phox)
5. These findings indicate that glycosylation and subsequent N-glycan maturation of Nox1 are both dispensable for its cell surface recruitment
6. Superoxide production by unglycosylated Nox1 is largely dependent on p22 (phox), which is abrogated by glutamine substitution for Pro-156 in p22 (phox), a mutation leading to a defective interaction with the Nox1-activating protein Noxo1
7. Thus p22 (phox) directly contributes to Nox1 activation in a glycosylation-independent manner, besides its significant role in Nox1 glycan maturation

Output (sent_index, trigger, protein, sugar, site):

• 0. glycosylation, , Nox1, -, -
• 2. glycosylation, , -, -, Asn-162 and Asn-236
• 2. glycosylation, , Nox1, -, Asn-162 and Asn-236
• 5. Nox1, , Nox1, subsequent N-glycan maturation, -
• 5. glycosylation, , Nox1, -, -
• 6. unglycosylated, , Nox1, -, -

Output(Part-Of) (sent_index, protein, site):

• 6. p22, Pro-156

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 2. Nox1, -, Asn-162 and Asn-236

 

Protein	NCBI ID	SENTENCE INDEX
Nox1	27035	0,1,2,3,4,5,6,7
Noxo1	124056	6
Thus p22	11261	7
p22	11261	3,4,6