PMID: 24417605

 

    Legend: Gene, Sites

Title : Glycomic analysis of high density lipoprotein shows a highly sialylated particle

Abstract :
  1. Many of the functional proteins and lipids in high density lipoprotein ( HDL ) particles are potentially glycosylated, yet very little is known about the glycoconjugates of HDL
  2. In this study, HDL was isolated from plasma by sequential micro-ultracentrifugation, followed by glycoprotein and glycolipid analysis
  3. N-Glycans, glycopeptides , and gangliosides were extracted and purified followed by analysis with nano-HPLC Chip quadrupole time of flight mass spectrometry and MS/MS
  4. HDL particles were found to be highly sialylated
  5. Most of the N-glycans (∼90%) from HDL glycoproteins were sialylated with one or two neuraminic acids (Neu5Ac)
  6. The most abundant N-glycan was a biantennary complex type glycan with two sialic acids (Hexose5HexNAc4Neu5Ac2) and was found in multiple glycoproteins using site-specific glycosylation analysis
  7. The observed O-glycans were all sialylated, and most contained a core 1 structure with two Neu5Acs, including those that were associated with apolipoprotein CIII ( ApoC-III ) and fetuin A. GM3 (monosialoganglioside, NeuAc2-3Gal1-4Glc-Cer) and GD3 (disialoganglioside, NeuAc2-8NeuAc2-3Gal1-4Glc-Cer) were the major gangliosides in HDL
  8. A 60% GM3 and 40% GD3 distribution was observed
  9. Both GM3 and GD3 were composed of heterogeneous ceramide lipid tails , including d18:1/16:0 and d18:1/23:0
  10. This report describes for the first time a glycomic approach for analyzing HDL , highlighting that HDL are highly sialylated particles
Output (sent_index, trigger, protein, sugar, site):
  • 1. glycosylated, , proteins, -, -
  • 10. sialylated, , HDL, -, -
  • 2. glycoprotein, , glycoprotein, -, -
  • 3. glycopeptides, , -, -, glycopeptides
  • 5. glycoproteins, , HDL glycoproteins, the N-glycans, -
  • 5. sialylated, , -, the N-glycans, -
  • 6. found, , glycoproteins, The most abundant N-glycan, -
  • 6. found, , glycoproteins, a biantennary complex type glycan, -
  • 6. glycoproteins, , glycoproteins, -, -
  • 7. apolipoprotein, , apolipoprotein CIII, fetuin A. GM3, -
  • 7. sialylated, , -, The observed O-glycans, -
Output(Part-Of) (sent_index, protein, site):
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
ApoC-III 345 7
apolipoprotein CIII 345 7