PMID: 24766575

 

    Legend: Gene, Sites

Title : Efficient and accurate glycopeptide identification pipeline for high-throughput site-specific N-glycosylation analysis

Abstract :

1. Study of site-specific N-glycosylation in complex sample remains a huge analytical challenge because protein glycosylation is structurally diverse in post-translational modifications, resulting in an intricacy of N-glycopeptides
2. Here we have developed a novel approach for high-throughput N-glycopeptide profiling based on a network-centric algorithm for deciphering glycan fragmentation in mass spectrometry
3. We performed an extensive validation and a high-throughput N-glycosylation study on serum and identified thousands of N-glycopeptide spectra with high confidence
4. The results revealed a similar level of glycan microheterogeneity to that of conventional glycomics approach on individual proteins and provided the unique in-depth site-specific information that could only be studied through glycopeptide profiling

Output (sent_index, trigger, protein, sugar, site):

• 0. glycopeptide, , -, -, glycopeptide
• 1. N-glycopeptides, , -, -, N-glycopeptides
• 2. N-glycopeptide, , -, -, N-glycopeptide
• 3. N-glycopeptide, , -, -, N-glycopeptide
• 4. glycopeptide, , -, -, glycopeptide

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX