PMID: 25081999

 

    Legend: Gene, Sites

Title : In vivo putative O-GlcNAcylation of human SCP1 and evidence for possible role of its N-terminal disordered structure

Abstract :

1. RNA polymerase II carboxyl-terminal domain (RNAPII CTD) phosphatases are responsible for the dephosphorylation of the C-terminal domain of the small subunit of RNAPII in eukaryotes
2. Recently, we demonstrated the identification of several interacting partners with human small CTD phosphatase1 ( hSCP1 ) and the substrate specificity to delineate an appearance of the dephosphorylation catalyzed by SCP1
3. In this study, using the established cells for inducibly expressing hSCP1 proteins , we monitored the modification of β-O-linked N-acetylglucosamine (O-GlcNAc)
4. O-GlcNAcylation is one of the most common post-translational modifications ( PTMs )
5. To gain insight into the PTM of hSCP1 , we used the Western blot, immunoprecipitation, succinylayed wheat germ agglutininprecipitation, liquid chromatography-mass spectrometry analyses, and site-directed mutagenesis and identified the Ser41 residue of hSCP1 as the O-GlcNAc modification site
6. These results suggest that hSCP1 may be an O-GlcNAcylated protein in vivo, and its N-terminus may function a possible role in the PTM, providing a scaffold for binding the protein (s)

Output (sent_index, trigger, protein, sugar, site):

• 0. SCP1, , SCP1, In vivo putative O-GlcNAcylation, -
• 5. Ser41, , -, the O-GlcNAc modification site, Ser41 residue
• 5. hSCP1, , hSCP1, the O-GlcNAc modification site, -

Output(Part-Of) (sent_index, protein, site):

• 1. RNA polymerase II, domain
• 1. subunit, domain
• 5. hSCP1, Ser41 residue

*Output_Site_Fusion* (sent_index, protein, sugar, site):

• 5. hSCP1, the O-GlcNAc modification site, Ser41 residue

 

Protein	NCBI ID	SENTENCE INDEX
hSCP1 proteins	58190	3
hSCP1	58190	2,5,6
SCP1	58190	0,2