PMID: 2550193

 

    Legend: Gene, Sites

Title : Recombinant human erythropoietin produced by Namalwa cells

Abstract :

1. To establish a practical exogenous gene expression system in human cells, a cDNA coding for human erythropoietin ( EPO ) was expressed in human B-lymphoblastoid Namalwa cells
2. The Namalwa-derived recombinant EPO was purified from the culture fluid by a simple three-step procedure
3. The Namalwa EPO showed an equivalent activity in vivo to that of human urinary EPO
4. Oligosaccharide structure analyses suggested that almost all N-linked oligosaccharide chains of Namalwa EPO are shared by urinary EPO
5. The two major N-linked oligosaccharides of Namalwa EPO were fucose-containing tetraantennary and fucose-containing triantennary structures

Output (sent_index, trigger, protein, sugar, site):

• 4. EPO, , Namalwa EPO, almost all N-linked oligosaccharide chains, -
• 5. EPO, , Namalwa EPO, The two major N-linked oligosaccharides, -

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
EPO	2056	1,2,3,4
Namalwa EPO	2056	3,4,5
erythropoietin	2056	0,1