PMID: 25759508

 

    Legend: Gene, Sites

Title : Hinge-Region O-Glycosylation of Human Immunoglobulin G3 ( IgG3 )

Abstract :
  1. Immunoglobulin G ( IgG ) is one of the most abundant proteins present in human serum and a fundamental component of the immune system
  2. IgG3 represents ∼8% of the total amount of IgG in human serum and stands out from the other IgG subclasses because of its elongated hinge region and enhanced effector functions
  3. This study reports partial O-glycosylation of the IgG3 hinge region , observed with nanoLC- ESI-IT-MS(/MS) analysis after proteolytic digestion
  4. The repeat regions within the IgG3 hinge were found to be in part O-glycosylated at the threonine in the triple repeat motif
  5. Non-, mono- and disialylated core 1-type O-glycans were detected in various IgG3 samples, both poly- and monoclonal
  6. NanoLC- ESI-IT-MS/MS with electron transfer dissociation fragmentation and CE-MS/MS with CID fragmentation were used to determine the site of IgG3 O-glycosylation
  7. The O-glycosylation site was further confirmed by the recombinant production of mutant IgG3 in which potential O-glycosylation sites had been knocked out
  8. For IgG3 samples from six donors we found similar O-glycan structures and site occupancies, whereas for the same samples the conserved N-glycosylation of the Fc CH2 domain showed considerable interindividual variation
  9. The occupancy of each of the three O-glycosylation sites was found to be ∼10% in six serum-derived IgG3 samples and ∼13% in two monoclonal IgG3 allotypes
Output (sent_index, trigger, protein, sugar, site):
  • 0. O-Glycosylation, , Human Immunoglobulin G3, -, -
  • 0. O-Glycosylation, , IgG3, -, -
  • 3. O-glycosylation, , -, -, region
  • 4. O-glycosylated, , -, -, -
  • 4. O-glycosylated, , -, -, threonine
  • 4. threonine, , -, The repeat regions, threonine
  • 4. threonine, , -, the triple repeat motif, threonine
  • 6. O-glycosylation, , -, -, site
  • 7. O-glycosylation, , -, -, site
  • 7. O-glycosylation, , -, -, sites
  • 8. N-glycosylation, , -, -, domain
  • 9. O-glycosylation, , -, -, sites
  • 9. occupancy, , -, -, sites
Output(Part-Of) (sent_index, protein, site):
  • 3. IgG3, region
*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

 

Protein NCBI ID SENTENCE INDEX
Human Immunoglobulin G3 3502 0
IgG3 3502 0,2,3,4,5,6,7