PMID: 25765764

 

    Legend: Gene, Sites

Title : LRIG1 extracellular domain : structure and function analysis

Abstract :

1. We have expressed and purified three soluble fragments of the human LRIG1- ECD (extracellular domain ): the LRIG1-LRR ( leucine-rich repeat) domain, the LRIG1-3Ig (immunoglobulin-like) domain , and the LRIG1- LRR-1 Ig fragment using baculovirus vectors in insect cells
2. The two LRIG1 domains crystallised so that we have been able to determine the three-dimensional structures at 2.3Å resolution
3. We developed a three-dimensional structure for the LRIG1- ECD using homology modelling based on the LINGO-1 structure
4. The LRIG1-LRR domain and the LRIG1-LRR-1Ig fragment are monomers in solution, whereas the LRIG1-3Ig domain appears to be dimeric
5. We could not detect any binding of the LRIG1 domains or the LRIG1-LRR-1Ig fragment to the EGF receptor ( EGFR ), either in solution using biosensor analysis or when the EGFR was expressed on the cell surface
6. The FLAG-tagged LRIG1-LRR-1Ig fragment binds weakly to colon cancer cells regardless of the presence of EGFRs
7. Similarly, neither the soluble LRIG1-LRR nor the LRIG1-3Ig domains nor the full-length LRIG1 co-expressed in HEK293 cells inhibited ligand-stimulated activation of cell-surface EGFR

Output (sent_index, trigger, protein, sugar, site):

• 1. LRIG1-ECD, , LRIG1, the LRIG1-LRR (leucine-rich repeat) domain, -
• 1. leucine-rich, , -, leucine-rich repeat, leucine
• 1. leucine-rich, , -, the LRIG1-LRR (leucine-rich repeat) domain, leucine

Output(Part-Of) (sent_index, protein, site):

• 0. LRIG1, domain
• 1. LRIG1, domain
• 1. LRIG1, fragment
• 1. LRIG1, fragments
• 2. LRIG1, domains
• 4. LRIG1, domain
• 4. LRIG1, fragment
• 5. LRIG1, domains
• 5. LRIG1, fragment
• 6. FLAG-tagged LRIG1, fragment
• 7. LRIG1, domains

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX
LINGO-1	84894	3
LRR-1	122769	1
LRIG1	26018	0,1,2,3,4,5,7
EGFR	1956	5,7
EGF receptor	1956	5
FLAG-tagged LRIG1	26018	6