PMID: 2590169

 

    Legend: Gene, Sites

Title : Dermatan sulphate proteoglycans of human articular cartilage

Abstract :

1. The properties of dermatan sulphate proteoglycans I and II
2. Dermatan sulphate proteoglycans were purified from juvenile human articular cartilage, with a yield of about 2 mg/g wet wt
3. of cartilage
4. Both dermatan sulphate proteoglycan I (DS- PGI ) and dermatan sulphate proteoglycan II (DS-PGII) were identified and the former was present in greater abundance
5. The two proteoglycans could not be resolved by agarose/polyacrylamide-gel electrophoresis, but could be resolved by SDS /polyacrylamide-gel electrophoresis, which indicated average Mr values of 200,000 and 98,000 for DS- PGI and DS-PGII respectively
6. After digestion with chondroitin ABC lyase the Mr values of the core proteins were 44,000 for DS- PGI and 43,000 and 47,000 for DS-PGII, with the smaller core protein being predominant in DS-PGII
7. Sequence analysis of the N-terminal 20 amino acid residues reveals the presence of a single site for the potential substitution of dermatan sulphate at residue 4 of DS-PGII and two such sites at residues 5 and 10 for DS- PGI

Output (sent_index, trigger, protein, sugar, site):

Output(Part-Of) (sent_index, protein, site):

*Output_Site_Fusion* (sent_index, protein, sugar, site):

 

Protein	NCBI ID	SENTENCE INDEX